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Tubing on a nano-liquid chromatography (nano-LC) technique, useful for very lower move capacities The inner diameter (ID) of an HPLC column is an important parameter.[44] It may influence the detection response when lessened because of the minimized lateral diffusion from the solute band. It may impact the separation selectivity, when circulation f
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This marriage decides the period of time it will eventually acquire for compound A to travel the length with the column. The greater time A spends adsorbed on the stationary phase, the greater time compound A will just take to journey the length in the column. The amount of time concerning the injection of a sample and its elution in the column is